Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99219

TMEM16A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TMEM16A rabbit pAb from ELK Biotechnology Read more ›

TMEM16A rabbit pAb
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TMEM16A rabbit pAb

Product Description

TMEM16A rabbit pAb is a research-use antibody from ELK Biotechnology for studying TMEM16A in IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

domain:The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region.,function:Acts as a calcium-activated chloride channel. Required for normal tracheal development.,similarity:Belongs to the anoctamin family.,tissue specificity:Broadly expressed with higher levels in liver and skeletal muscle.,

Additional antibody information

Specificity TMEM16A Polyclonal Antibody detects endogenous levels of TMEM16A protein.
Validation evidence captions Immunofluorescence analysis of HeLa cells, using TM16A Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: TMEM16A
Collaborator ELK Biotechnology
Partner model ES6706
Target TMEM16A
Research areasAntibody Research
Reactivity Human,Mouse
Applications IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human TM16A. AA range:921-970
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Broadly expressed with higher levels in liver, skeletal muscle and gastrointestinal muscles.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
Human Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TMEM16A
Also known as
ANO1; DOG1; ORAOV2; TAOS2; TMEM16A; Anoctamin-1; Discovered on gastrointestinal stromal tumors protein 1; Oral cancer overexpressed protein 2; Transmembrane protein 16A; Tumor-amplified and overexpressed sequence 2
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TM16A. AA range:921-970
Cellular localization
Cell membrane ; Multi-pass membrane protein . Cytoplasm . Cytoplasmic localization seen in neoplastic cells of head and neck squamous cell carcinoma (HNSCC) tumors. .
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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