ABM99217
GPR172B rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
GPR172B rabbit pAb from ELK Biotechnology Read more ›
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Product Description
GPR172B rabbit pAb is an ELK Biotechnology antibody for research workflows involving GPR172B.
Background
Biological redox reactions require electron donors and acceptor. Vitamin B2 is the source for the flavin in flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) which are common redox reagents. This gene encodes a member of the riboflavin (vitamin B2) transporter family. Haploinsufficiency of this protein can cause maternal riboflavin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2013],
Additional antibody information
| Specificity | GPR172B Polyclonal Antibody detects endogenous levels of GPR172B protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T cells using GPR172B Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of MCF7 cells, using PEVR2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from LOVO cells, using PEVR2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using PEVR2 antibody. |
Key Facts
Product type
Primary Antibody›
Target: GPR172B
| Target | GPR172B |
| Also known as | SLC52A1; GPR172B; PAR2; RFT1; Solute carrier family 52; riboflavin transporter, member 1; Porcine endogenous retrovirus A receptor 2; PERV-A receptor 2; Protein GPR172B; Riboflavin transporter 1; hRFT1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 46kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PEVR2. AA range:235-284 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
GPR172B
Also known as
SLC52A1; GPR172B; PAR2; RFT1; Solute carrier family 52; riboflavin transporter, member 1; Porcine endogenous retrovirus A receptor 2; PERV-A receptor 2; Protein GPR172B; Riboflavin transporter 1; hRFT1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PEVR2. AA range:235-284
Observed band
46kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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