Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99211

CDKAL1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CDKAL1 rabbit pAb from ELK Biotechnology Read more ›

CDKAL1 rabbit pAb
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CDKAL1 rabbit pAb

Product Description

CDKAL1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CDKAL1 in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes. [provided by RefSeq, May 2010],

Additional antibody information

Specificity CDKAL1 Polyclonal Antibody detects endogenous levels of CDKAL1 protein.
Validation evidence captions Western Blot analysis of CoLo cells using CDKAL1 Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: CDKAL1
Collaborator ELK Biotechnology
Partner model ES6681
Target CDKAL1
Research areasAntibody Research
Reactivity Human,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CDKAL. AA range:10-59
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in pancreatic islets.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CDKAL1
Also known as
CDKAL1; Threonylcarbamoyladenosine tRNA methylthiotransferase; CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CDKAL. AA range:10-59
Observed band
65kD
Cellular localization
Endoplasmic reticulum membrane ; Single-pass membrane protein . Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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