PC-PLD1 (phospho Ser561) rabbit pAb from ELK Biotechnology Read more ›
PC-PLD1 (phospho Ser561) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PC-PLD1 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],
| Specificity | Phospho-PC-PLD1 (S561) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at S561. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLD1 (Phospho-Ser561) Antibody | Immunofluorescence analysis of HepG2 cells, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6612 |
| Target | PC-PLD1 |
| Research areas | >>Glycerophospholipid metabolism, >>Ether lipid metabolism, >>Metabolic pathways, >>Ras signaling pathway, >>cAMP signaling pathway, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Endocytosis, >>Fc gamma R-mediated phagocytosis, >>Glutamatergic synapse, >>GnRH signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Pathways in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Pancreatic cancer, >>Choline metabolism in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLD1 around the phosphorylation site of Ser561. AA range:527-576 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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