Manufactured by:ELK Biotechnology
RUO

PC-PLD1 (phospho Thr147) rabbit pAb

PC-PLD1 (phospho Thr147) rabbit pAb from ELK Biotechnology

SKU:
ABM109046
Product type
Primary Antibody
TargetPC-PLD1
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

PC-PLD1 (phospho Thr147) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PC-PLD1 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],

Additional antibody information

Specificity Phospho-PC-PLD1 (T147) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at T147.
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000
Key facts & specifications
Research areas
>>Glycerophospholipid metabolism, >>Ether lipid metabolism, >>Metabolic pathways, >>Ras signaling pathway, >>cAMP signaling pathway, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Endocytosis, >>Fc gamma R-mediated phagocytosis, >>Glutamatergic synapse, >>GnRH signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Pathways in cancer, >>Chemical carcinogenesis - reactive oxygen species, >>Pancreatic cancer, >>Choline metabolism in cancer
Also known as
PLD1; Phospholipase D1; PLD 1; hPLD1; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human PC-PLD1 (phospho Thr147)
Observed band
120kD
Cellular localization
Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES6611
UniProt entry
Q13393
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00