ABM99162
Endomucin rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Endomucin rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Endomucin rabbit pAb is an ELK Biotechnology antibody for research workflows involving Endomucin.
Background
endomucin(EMCN) Homo sapiens EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008],
Additional antibody information
| Specificity | Endomucin Polyclonal Antibody detects endogenous levels of Endomucin protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HepG2 cells, using Mucin-14 Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: Endomucin
| Target | Endomucin |
| Also known as | EMCN; EMCN2; MUC14; Endomucin; Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14 |
| Concentration | 1 mg/ml |
| Working concentration | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Cellular localization | Isoform 1: Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.; Isoform 2: Secreted . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IFELISA |
| Dilution range | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Mucin-14. AA range:201-250 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Endomucin
Also known as
EMCN; EMCN2; MUC14; Endomucin; Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Mucin-14. AA range:201-250
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.; Isoform 2: Secreted .
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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