Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99162

Endomucin rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Endomucin rabbit pAb from ELK Biotechnology Read more ›

Endomucin rabbit pAb 50µL/100µL
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Endomucin rabbit pAb 50µL/100µL

Product Description

Endomucin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Endomucin in IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

endomucin(EMCN) Homo sapiens EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008],

Additional antibody information

Specificity Endomucin Polyclonal Antibody detects endogenous levels of Endomucin protein.
Validation evidence captions Immunofluorescence analysis of HepG2 cells, using Mucin-14 Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: Endomucin
Collaborator ELK Biotechnology
Partner model ES6559
Target Endomucin
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human Mucin-14. AA range:201-250
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in heart, kidney and lung.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
Human Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Endomucin
Also known as
EMCN; EMCN2; MUC14; Endomucin; Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Mucin-14. AA range:201-250
Cellular localization
Isoform 1: Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.; Isoform 2: Secreted .
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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