PDGFR-β (phospho Tyr771) rabbit pAb
PDGFR-β (phospho Tyr771) rabbit pAb from ELK Biotechnology Read more ›
Product Description
PDGFR-β (phospho Tyr771) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PDGFR-β.
Background
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-PDGFR-β (Y771) Polyclonal Antibody detects endogenous levels of PDGFR-β protein only when phosphorylated at Y771. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of mouse lung , rat lung,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | PDGFR-β |
| Also known as | PDGFRB; PDGFR; PDGFR1; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-deri |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Rat |
| Observed band | 135-180kD |
| Cellular localization | Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>Phospholipase D signaling pathway;>>PI3K-Akt signaling pathway;>>Focal adhesion;>>Gap junction;>>JAK-STAT signaling pathway;>>Regulation of actin cytoskeleton;>>Human papillomavirus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Central carbon metabolism in cancer;>>Choline metabolism in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human PDGFR-β (phospho Tyr771) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.