PDGFR-β (phospho Tyr771) rabbit pAb from ELK Biotechnology Read more ›
PDGFR-β (phospho Tyr771) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PDGFR-β in WB, IHC workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-PDGFR-β (Y771) Polyclonal Antibody detects endogenous levels of PDGFR-β protein only when phosphorylated at Y771. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Western Blot analysis of mouse lung , rat lung,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES6541 |
| Target | PDGFR-β |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>Phospholipase D signaling pathway, >>PI3K-Akt signaling pathway, >>Focal adhesion, >>Gap junction, >>JAK-STAT signaling pathway, >>Regulation of actin cytoskeleton, >>Human papillomavirus infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Glioma, >>Prostate cancer, >>Melanoma, >>Central carbon metabolism in cancer, >>Choline metabolism in cancer |
| Reactivity | Human,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human PDGFR-β (phospho Tyr771) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Spleen, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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