V-ATPase D rabbit pAb from ELK Biotechnology Read more ›
V-ATPase D rabbit pAb is a research-use antibody from ELK Biotechnology for studying V-ATPase D in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Swine. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008],
| Specificity | V-ATPase D Polyclonal Antibody detects endogenous levels of V-ATPase D protein. |
|---|---|
| Validation evidence captions | Western blot analysis of ATP6V1D Antibody. The lane on the right is blocked with the ATP6V1D peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6522 |
| Target | V-ATPase D |
| Research areas | >>Oxidative phosphorylation, >>Metabolic pathways, >>Phagosome, >>mTOR signaling pathway, >>Synaptic vesicle cycle, >>Collecting duct acid secretion, >>Vibrio cholerae infection, >>Epithelial cell signaling in Helicobacter pylori infection, >>Human papillomavirus infection, >>Rheumatoid arthritis |
| Reactivity | Human,Mouse,Rat,Swine |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from V-ATPase D . at AA range: 70-150 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Bone marrow,Brain,Heart,Pancreatic adenocarcinoma,Pituitary,Placent |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Swine |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Swine | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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