PIPOX rabbit pAb from ELK Biotechnology Read more ›
PIPOX rabbit pAb is a research-use antibody from ELK Biotechnology for studying PIPOX in WB, IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:L-pipecolate + O(2) = 2,3,4,5-tetrahydropyridine-2-carboxylate + H(2)O(2).,catalytic activity:Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2).,cofactor:Binds 1 FAD per subunit.,function:Metabolizes sarcosine, L-pipecolic acid and L-proline.,similarity:Belongs to the MSOX/MTOX family.,subunit:Monomer.,
| Specificity | PIPOX Polyclonal Antibody detects endogenous levels of PIPOX protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using PIPOX Polyclonal Antibody | Western blot analysis of lysates from NIH/3T3 and RAW264.7 cells, using PIPOX Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES6510 |
| Target | PIPOX |
| Research areas | >>Glycine, serine and threonine metabolism, >>Lysine degradation, >>Metabolic pathways, >>Peroxisome |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PIPOX. AA range:257-306 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in the liver and kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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