Elongin A2/3 rabbit pAb
Elongin A2/3 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Elongin A2/3 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Elongin A2/3.
Background
This gene encodes the transcriptionally active subunit of the SIII (or elongin) transcription elongation factor complex, which also includes two regulatory subunits, elongins B and C. This complex acts to increase the rate of RNA chain elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites along the DNA template. Whereas a related protein with similar function, elongin A, is ubiquitously expressed, the encoded protein is specifically expressed in the testis, suggesting it may have a role in spermatogenesis. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Elongin A2/3 Polyclonal Antibody detects endogenous levels of Elongin A2/3 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using ELOA2 Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Elongin A2/3 |
| Also known as | TCEB3B; TCEB3L; RNA polymerase II transcription factor SIII subunit A2; Elongin-A2; EloA2; Transcription elongation factor B polypeptide 3B; TCEB3C; TCEB3L2; RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcr |
| Concentration | 1 mg/ml |
| Working concentration | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Cellular localization | Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IFELISA |
| Dilution range | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ELOA2. AA range:101-150 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.