Wee 2 rabbit pAb from ELK Biotechnology Read more ›
Wee 2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Wee 2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Phosphorylates and inhibits CDC2. May act as a negative regulator of entry into mitosis (G2 to M transition).,PTM:Phosphorylated .,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed in testis.,
| Specificity | Wee 2 Polyclonal Antibody detects endogenous levels of Wee 2 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from HUVEC cells, using WEE2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using WEE2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6439 |
| Target | Wee 2 |
| Research areas | >>Cell cycle, >>Human immunodeficiency virus 1 infection |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human WEE2. AA range:151-200 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in oocytes (at protein level) (PubMed:29606300). May also be expressed in testis (PubMed:11029659). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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