NSF rabbit pAb
NSF rabbit pAb from ELK Biotechnology Read more ›
Product Description
NSF rabbit pAb is an ELK Biotechnology antibody for research workflows involving NSF.
Background
catalytic activity:ATP + H(2)O = ADP + phosphate.,cofactor:Binds 1 magnesium ion per subunit.,function:Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.,similarity:Belongs to the AAA ATPase family.,subunit:Homohexamer. Interacts with GABARAP and GABARAPL2.,
Additional antibody information
| Specificity | NSF Polyclonal Antibody detects endogenous levels of NSF protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of CoLo cells using NSF Polyclonal Antibody |
Key Facts
| Target | NSF |
| Also known as | NSF; Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 75kD |
| Cellular localization | Cytoplasm. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Synaptic vesicle cycle;>>GABAergic synapse;>>Vasopressin-regulated water reabsorption |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from NSF . at AA range: 110-190 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.