NSF rabbit pAb from ELK Biotechnology Read more ›
NSF rabbit pAb is a research-use antibody from ELK Biotechnology for studying NSF in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:ATP + H(2)O = ADP + phosphate.,cofactor:Binds 1 magnesium ion per subunit.,function:Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.,similarity:Belongs to the AAA ATPase family.,subunit:Homohexamer. Interacts with GABARAP and GABARAPL2.,
| Specificity | NSF Polyclonal Antibody detects endogenous levels of NSF protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of CoLo cells using NSF Polyclonal Antibody |
| Collaborator | ELK Biotechnology |
| Partner model | ES6419 |
| Target | NSF |
| Research areas | >>Synaptic vesicle cycle, >>GABAergic synapse, >>Vasopressin-regulated water reabsorption |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from NSF . at AA range: 110-190 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Kidney,Lung, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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