Cleaved-Notch 4 (V1432) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Notch 4 (V1432) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Notch 4 in WB, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
notch 4(NOTCH4) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which
| Specificity | Cleaved-Notch 4 (V1432) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 4 protein resulting from cleavage adjacent to V1432. |
|---|---|
| Validation evidence captions | Western Blot analysis of A549 NIH-3T3 SH-SY5Y K562 HELA cells using Cleaved-Notch 4 (V1432) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from 293 and COS7 cells, treated with etoposide 25uM 1h, using NOTCH4 (Cleaved-Val1432) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6409 |
| Target | Notch 4 |
| Research areas | >>Endocrine resistance, >>Notch signaling pathway, >>Thyroid hormone signaling pathway, >>Human papillomavirus infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Breast cancer |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NOTCH4. AA range:1401-1450 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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