Cleaved-Notch 2 (A1734) rabbit pAb
Cleaved-Notch 2 (A1734) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-Notch 2 (A1734) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Notch 2.
Background
notch 2(NOTCH2) Homo sapiens This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cle
Additional antibody information
| Specificity | Cleaved-Notch 2 (A1734) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to A1734. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using Cleaved-Notch 2 (A1734) Polyclonal Antibody diluted at 1:2000 | Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using NOTCH2 (Cleaved-Ala1734) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Notch 2 |
| Also known as | NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 110(cleaved)kD |
| Cellular localization | Notch 2 extracellular truncation: Cell membrane ; Single-pass type I membrane protein .; Notch 2 intracellular domain: Nucleus . Cytoplasm . Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by TCIM (PubMed:25985737). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocrine resistance;>>Notch signaling pathway;>>Th1 and Th2 cell differentiation;>>Thyroid hormone signaling pathway;>>Human papillomavirus infection;>>Pathways in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Breast cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NOTCH2. AA range:1715-1764 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.