Cleaved-Notch 2 (A1734) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Notch 2 (A1734) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Notch 2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
notch 2(NOTCH2) Homo sapiens This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cle
| Specificity | Cleaved-Notch 2 (A1734) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to A1734. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using Cleaved-Notch 2 (A1734) Polyclonal Antibody diluted at 1:2000 | Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using NOTCH2 (Cleaved-Ala1734) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6407 |
| Target | Notch 2 |
| Research areas | >>Endocrine resistance, >>Notch signaling pathway, >>Th1 and Th2 cell differentiation, >>Thyroid hormone signaling pathway, >>Human papillomavirus infection, >>Pathways in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Breast cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NOTCH2. AA range:1715-1764 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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