eNOS (phospho Ser615) rabbit pAb from ELK Biotechnology Read more ›
eNOS (phospho Ser615) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NOS3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
| Specificity | Phospho-NOS3 (S615) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S615. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eNOS (Phospho-Ser615) Antibody | Western blot analysis of lysates from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6400 |
| Target | NOS3 |
| Research areas | >>Arginine biosynthesis, >>Arginine and proline metabolism, >>Metabolic pathways, >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>HIF-1 signaling pathway, >>Sphingolipid signaling pathway, >>PI3K-Akt signaling pathway, >>VEGF signaling pathway, >>Apelin signaling pathway, >>Platelet activation, >>Estrogen signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Insulin resistance, >>AGE-RAGE signaling pathway in diabetic complications, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser615. AA range:581-630 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Platelets, placenta, liver and kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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