eNOS (phospho Thr494) rabbit pAb from ELK Biotechnology Read more ›
eNOS (phospho Thr494) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NOS3/eNOS in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],
| Specificity | Phospho-NOS3 (T494) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at T494. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using Phospho-NOS3 (T494) Polyclonal Antibody diluted at 1:1000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eNOS (Phospho-Thr494) Antibody | Western blot analysis of lysates from Jurkat cells, using eNOS (Phospho-Thr494) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6399 |
| Target | NOS3/eNOS |
| Research areas | >>Arginine biosynthesis, >>Arginine and proline metabolism, >>Metabolic pathways, >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>HIF-1 signaling pathway, >>Sphingolipid signaling pathway, >>PI3K-Akt signaling pathway, >>VEGF signaling pathway, >>Apelin signaling pathway, >>Platelet activation, >>Estrogen signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Insulin resistance, >>AGE-RAGE signaling pathway in diabetic complications, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Thr494. AA range:462-511 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Platelets, placenta, liver and kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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