Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99098

Nkx-6.1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Nkx-6.1 rabbit pAb from ELK Biotechnology Read more ›

Nkx-6.1 rabbit pAb
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Nkx-6.1 rabbit pAb

Product Description

Nkx-6.1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Nkx-6.1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008],

Additional antibody information

Specificity Nkx-6.1 Polyclonal Antibody detects endogenous levels of Nkx-6.1 protein.
Validation evidence captions Western blot analysis of NKX61 Antibody. The lane on the right is blocked with the NKX61 peptide.

Key Facts

Product typePrimary Antibody Target: Nkx-6.1
Collaborator ELK Biotechnology
Partner model ES6394
Target Nkx-6.1
Research areas>>Maturity onset diabetes of the young
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Nkx-6.1 . at AA range: 180-260
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Pancreatic beta cells.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Nkx-6.1
Also known as
NKX6-1; NKX6A; Homeobox protein Nkx-6.1; Homeobox protein NK-6 homolog A
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from Nkx-6.1 . at AA range: 180-260
Cellular localization
Nucleus .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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