NFκB-p100 (phospho Ser872) rabbit pAb from ELK Biotechnology Read more ›
NFκB-p100 (phospho Ser872) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NF-κB p100/p52 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
| Specificity | Phospho-NFκB-p100 (S872) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S872. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 (Phospho-Ser872) Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(Immunoway cat:RS23920)was diluted at 1:10000, 37° 1hour. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6379 |
| Target | NF-κB p100/p52 |
| Research areas | >>MAPK signaling pathway, >>NF-kappa B signaling pathway, >>Osteoclast differentiation, >>C-type lectin receptor signaling pathway, >>Legionellosis, >>Human T-cell leukemia virus 1 infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Viral carcinogenesis, >>Breast cancer |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p100 around the phosphorylation site of Ser872. AA range:838-887 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Leukemia,Lymph,Thymus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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