NFκB-p100 (phospho Ser872) rabbit pAb
NFκB-p100 (phospho Ser872) rabbit pAb from ELK Biotechnology Read more ›
Product Description
NFκB-p100 (phospho Ser872) rabbit pAb is an ELK Biotechnology antibody for research workflows involving NF-κB p100/p52.
Background
nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
Additional antibody information
| Specificity | Phospho-NFκB-p100 (S872) Polyclonal Antibody detects endogenous levels of NFκB-p100 protein only when phosphorylated at S872. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 (Phospho-Ser872) Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(Immunoway cat:RS23920)was diluted at 1:10000, 37° 1hour. |
Key Facts
| Target | NF-κB p100/p52 |
| Also known as | NFKB2; LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 96kD |
| Cellular localization | Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Osteoclast differentiation;>>C-type lectin receptor signaling pathway;>>Legionellosis;>>Human T-cell leukemia virus 1 infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Viral carcinogenesis;>>Breast cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p100 around the phosphorylation site of Ser872. AA range:838-887 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.