Na+/K+-ATPase α2 rabbit pAb from ELK Biotechnology Read more ›
Na+/K+-ATPase α2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Na+/K+-ATPase α2 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008],
| Specificity | Na+/K+-ATPase α2 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α2 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from COS7 cells, HepG2 cells, and Jurkat cells, using ATP1A2 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES6362 |
| Target | Na+/K+-ATPase α2 |
| Research areas | >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Cardiac muscle contraction, >>Adrenergic signaling in cardiomyocytes, >>Insulin secretion, >>Thyroid hormone synthesis, >>Thyroid hormone signaling pathway, >>Aldosterone synthesis and secretion, >>Aldosterone-regulated sodium reabsorption, >>Endocrine and other factor-regulated calcium reabsorption, >>Proximal tubule bicarbonate reclamation, >>Salivary secretion, >>Gastric acid secretion, >>Pancreatic secretion, >>Carbohydrate digestion and absorption, >>Protein digestion and absorption, >>Bile secretion, >>Mineral absorption |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATP1A2. AA range:971-1020 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Leukocyte,Ovary,Placenta,Uterus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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