Na+/K+-ATPase α2 rabbit pAb
Na+/K+-ATPase α2 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Na+/K+-ATPase α2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Na+/K+-ATPase α2.
Background
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008],
Additional antibody information
| Specificity | Na+/K+-ATPase α2 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α2 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from COS7 cells, HepG2 cells, and Jurkat cells, using ATP1A2 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | Na+/K+-ATPase α2 |
| Also known as | ATP1A2; KIAA0778; Sodium/potassium-transporting ATPase subunit alpha-2; Na(+)/K(+) ATPase alpha-2 subunit; Sodium pump subunit alpha-2 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat;Monkey |
| Observed band | 112kD |
| Cellular localization | Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Cardiac muscle contraction;>>Adrenergic signaling in cardiomyocytes;>>Insulin secretion;>>Thyroid hormone synthesis;>>Thyroid hormone signaling pathway;>>Aldosterone synthesis and secretion;>>Aldosterone-regulated sodium reabsorption;>>Endocrine and other factor-regulated calcium reabsorption;>>Proximal tubule bicarbonate reclamation;>>Salivary secretion;>>Gastric acid secretion;>>Pancreatic secretion;>>Carbohydrate digestion and absorption;>>Protein digestion and absorption;>>Bile secretion;>>Mineral absorption |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATP1A2. AA range:971-1020 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.