NDUFS3 rabbit pAb from ELK Biotechnology Read more ›
NDUFS3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NDUFS3 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009],
| Specificity | NDUFS3 Polyclonal Antibody detects endogenous levels of NDUFS3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T cells using NDUFS3 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from mouse brain, using NDUFS3 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using NDUFS3 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6350 |
| Target | NDUFS3 |
| Research areas | >>Oxidative phosphorylation, >>Metabolic pathways, >>Thermogenesis, >>Retrograde endocannabinoid signaling, >>Non-alcoholic fatty liver disease, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Chemical carcinogenesis - reactive oxygen species, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NDUFS3. AA range:117-166 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Cajal-Retzius cell,Pituitary,Skin,Stomach mucosa,Uter |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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