Nibrin (phospho Ser343) rabbit pAb from ELK Biotechnology Read more ›
Nibrin (phospho Ser343) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Nibrin in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Nibrin (S343) Polyclonal Antibody detects endogenous levels of Nibrin protein only when phosphorylated at S343. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jurkat cells, using p95/NBS1 (Phospho-Ser343) Antibody. The lane on the left is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6334 |
| Target | Nibrin |
| Research areas | >>Homologous recombination, >>Cellular senescence |
| Reactivity | Human,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p95/NBS1 around the phosphorylation site of Ser343. AA range:310-359 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous (PubMed:9590180). Expressed at high levels in testis (PubMed:9590180). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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