Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99079

CREB-2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CREB-2 rabbit pAb from ELK Biotechnology Read more ›

CREB-2 rabbit pAb
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CREB-2 rabbit pAb

Product Description

CREB-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CREB-2 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

activating transcription factor 4(ATF4) Homo sapiens This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provid

Additional antibody information

Specificity CREB-2 Polyclonal Antibody detects endogenous levels of CREB-2 protein.
Validation evidence captions Western Blot analysis of A549 cells using CREB-2 Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: CREB-2
Collaborator ELK Biotechnology
Partner model ES6333
Target CREB-2
Research areas>>MAPK signaling pathway, >>cGMP-PKG signaling pathway, >>Mitophagy - animal, >>Protein processing in endoplasmic reticulum, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Longevity regulating pathway, >>Adrenergic signaling in cardiomyocytes, >>TNF signaling pathway, >>Long-term potentiation, >>Neurotrophin signaling pathway, >>Cholinergic synapse, >>Dopaminergic synapse, >>Insulin secretion, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Thyroid hormone synthesis, >>Glucagon signaling pathway, >>Aldosterone synthesis and secretion, >>Relaxin signaling pathway, >>Cortisol synthesis and secretion, >>Parathyroid hormone synthesis, secretion and action, >>Non-alcoholic fatty liver disease, >>Cushing syndrome, >>Growth hormone synthesis, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Cocaine addiction, >>Amphetamine addiction, >>Alcoholism, >>Hepatitis B, >>Human cytomegalovirus infection, >>Hu
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from CREB-2 . at AA range: 160-240
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Bladder,Colon,Fibroblast,Leukemic T-cell,Lung,Ovary,Placenta,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CREB-2
Also known as
ATF4; CREB2; TXREB; Cyclic AMP-dependent transcription factor ATF-4; cAMP-dependent transcription factor ATF-4; Activating transcription factor 4; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding prot
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from CREB-2 . at AA range: 160-240
Observed band
38kD
Cellular localization
Nucleus . Nucleus speckle . Cytoplasm . Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Colocalizes with GABBR1 in hippocampal neuron dendritic membranes (By similarity). Colocalizes with NEK6 at the centrosome (PubMed:20873783). Recruited to nuclear speckles following interaction with EP300/p300 (PubMed:16219772). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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