MYLK rabbit pAb
MYLK rabbit pAb from ELK Biotechnology Read more ›
Product Description
MYLK rabbit pAb is an ELK Biotechnology antibody for research workflows involving MYLK.
Background
myosin light chain kinase(MYLK) Homo sapiens This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been
Additional antibody information
| Specificity | MYLK Polyclonal Antibody detects endogenous levels of MYLK protein. |
|---|---|
| Validation evidence captions | Western blot analysis of the lysates from HT-29 cells using MYLK antibody. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
| Target | MYLK |
| Also known as | MYLK; MLCK; MLCK1; MYLK1; Myosin light chain kinase; smooth muscle; MLCK; smMLCK; Kinase-related protein; KRP; Telokin |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 200kD |
| Cellular localization | Cytoplasm . Cell projection, lamellipodium . Cleavage furrow . Cytoplasm, cytoskeleton, stress fiber . Localized to stress fibers during interphase and to the cleavage furrow during mitosis. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>Vascular smooth muscle contraction;>>Apelin signaling pathway;>>Focal adhesion;>>Platelet activation;>>Regulation of actin cytoskeleton;>>Oxytocin signaling pathway;>>Gastric acid secretion |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MYLK. AA range:1701-1750 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.