Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99070

MxB rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MxB rabbit pAb from ELK Biotechnology Read more ›

MxB rabbit pAb
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MxB rabbit pAb

Product Description

MxB rabbit pAb is a research-use antibody from ELK Biotechnology for studying MxB in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity MxB Polyclonal Antibody detects endogenous levels of MxB protein.
Validation evidence captions Western Blot analysis of 293 cells using MxB Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: MxB
Collaborator ELK Biotechnology
Partner model ES6295
Target MxB
Research areas>>Viral life cycle - HIV-1, >>Hepatitis C, >>Measles, >>Influenza A, >>Human papillomavirus infection, >>Coronavirus disease - COVID-19
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from the Internal region of human MxB.
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
MxB
Also known as
MX2; Interferon-induced GTP-binding protein Mx2; Interferon-regulated resistance GTP-binding protein MxB; Myxovirus resistance protein 2; p78-related protein
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from the Internal region of human MxB.
Observed band
82kD
Cellular localization
Cytoplasm . Nucleus . Nucleus, nuclear pore complex . Localization to nuclear pores requires GTP-binding.
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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