Moesin/Ezrin/Radixin (phospho Thr558) rabbit pAb from ELK Biotechnology Read more ›
Moesin/Ezrin/Radixin (phospho Thr558) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Moesin/Ezrin/Radixin in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Moesin/Ezrin/Radixin (T558) Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein only when phosphorylated at T558. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human pancreas, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from NIH/3T3 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6283 |
| Target | Moesin/Ezrin/Radixin |
| Research areas | >>Tight junction, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Measles, >>Proteoglycans in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558. AA range:524-573 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | In all tissues and cultured cells studied. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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