ABM99052
MLF1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
MLF1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
MLF1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving MLF1.
Background
myeloid leukemia factor 1(MLF1) Homo sapiens This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010],
Additional antibody information
| Specificity | MLF1 Polyclonal Antibody detects endogenous levels of MLF1 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of MLF1 Antibody. The lane on the right is blocked with the MLF1 peptide. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: MLF1
| Target | MLF1 |
| Also known as | MLF1; Myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse |
| Observed band | 30kD |
| Cellular localization | Cytoplasm . Nucleus . Cell projection, cilium . Cytoplasm, cytoskeleton, cilium basal body . Shuttles between the cytoplasm and nucleus. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Transcriptional misregulation in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | Synthesized peptide derived from MLF1 . at AA range: 130-210 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
MLF1
Also known as
MLF1; Myeloid leukemia factor 1; Myelodysplasia-myeloid leukemia factor 1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from MLF1 . at AA range: 130-210
Observed band
30kD
Cellular localization
Cytoplasm . Nucleus . Cell projection, cilium . Cytoplasm, cytoskeleton, cilium basal body . Shuttles between the cytoplasm and nucleus. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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