CD71/TfR (phospho Ser24) rabbit pAb from ELK Biotechnology Read more ›
CD71/TfR (phospho Ser24) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD71/TfR in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
| Specificity | Phospho-CD71 (S24) Polyclonal Antibody detects endogenous levels of CD71 protein only when phosphorylated at S24. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using Phospho-CD71 (S24) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD71/TfR (Phospho-Ser24) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using CD71/TfR (Phospho-Ser24) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' , using CD71/TfR (Phospho-Ser24) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES6200 |
| Target | CD71/TfR |
| Research areas | >>HIF-1 signaling pathway, >>Endocytosis, >>Phagosome, >>Ferroptosis, >>Hematopoietic cell lineage |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD71/TfR around the phosphorylation site of Ser24. AA range:15-64 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium,Erythroleukemia,Eye,Human endometrium carcinoma cell line,Liver,Pl |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
