Cleaved-Kininogen-1 HC (K380) rabbit pAb
Cleaved-Kininogen-1 HC (K380) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-Kininogen-1 HC (K380) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Kininogen 1.
Background
This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014],
Additional antibody information
| Specificity | Cleaved-Kininogen-1 HC (K380) Polyclonal Antibody detects endogenous levels of fragment of activated Kininogen-1 HC protein resulting from cleavage adjacent to K380. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using Cleaved-Kininogen-1 HC (K380) Polyclonal Antibody |
Key Facts
| Target | Kininogen 1 |
| Also known as | KNG1; BDK; KNG; Kininogen-1; Alpha-2-thiol proteinase inhibitor; Fitzgerald factor; High molecular weight kininogen; HMWK; Williams-Fitzgerald-Flaujeac factor |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 41kD |
| Cellular localization | Secreted, extracellular space. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>cGMP-PKG signaling pathway;>>Sphingolipid signaling pathway;>>Neuroactive ligand-receptor interaction;>>Complement and coagulation cascades;>>Inflammatory mediator regulation of TRP channels;>>Regulation of actin cytoskeleton;>>Chagas disease;>>African trypanosomiasis;>>Pathways in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from Cleaved-Kininogen-1 HC (K380) . at AA range: 300-380 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.