IRS-1 (phospho Tyr896) rabbit pAb from ELK Biotechnology Read more ›
IRS-1 (phospho Tyr896) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IRS-1 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],
| Specificity | Phospho-IRS-1 (Y896) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Y896. |
|---|---|
| Validation evidence captions | Western blot analysis of KB MCF-7 using p-IRS-1 (Y896) antibody. Antibody was diluted at 1:500 | Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using IRS-1 (Phospho-Tyr896) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES5926 |
| Target | IRS-1 |
| Research areas | >>cGMP-PKG signaling pathway, >>FoxO signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Neurotrophin signaling pathway, >>Insulin signaling pathway, >>Adipocytokine signaling pathway, >>Regulation of lipolysis in adipocytes, >>Type II diabetes mellitus, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>Growth hormone synthesis, secretion and action, >>Aldosterone-regulated sodium reabsorption, >>Alzheimer disease, >>MicroRNAs in cancer, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Tyr896. AA range:862-911 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Epithelium,Eye,Skeletal muscle, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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