IL-13Rα1 (phospho Tyr405) rabbit pAb from ELK Biotechnology Read more ›
IL-13Rα1 (phospho Tyr405) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-13Rα1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-IL-13Rα1 (Y405) Polyclonal Antibody detects endogenous levels of IL-13Rα1 protein only when phosphorylated at Y405. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Phospho-IL-13Rα1 (Y405) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IL-13R alpha1 (Phospho-Tyr405) Antibody | Immunofluorescence analysis of HepG2 cells, using IL-13R alpha1 (Phospho-Tyr405) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HUVEC cells treated with serum 20% 15', using IL-13R alpha1 (Phospho-Tyr405) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5896 |
| Target | IL-13Rα1 |
| Research areas | >>Cytokine-cytokine receptor interaction, >>JAK-STAT signaling pathway, >>Pathways in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-13R alpha1 around the phosphorylation site of Tyr405. AA range:371-420 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
