IL-7R (phospho Tyr449) rabbit pAb from ELK Biotechnology Read more ›
IL-7R (phospho Tyr449) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-7R in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015],
| Specificity | Phospho-IL-7R (Y449) Polyclonal Antibody detects endogenous levels of IL-7R protein only when phosphorylated at Y449. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HUVEC cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from COLO205 cells, using IL-7R/CD127 (Phospho-Tyr449) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5884 |
| Target | IL-7R |
| Research areas | >>Cytokine-cytokine receptor interaction, >>FoxO signaling pathway, >>PI3K-Akt signaling pathway, >>JAK-STAT signaling pathway, >>Hematopoietic cell lineage, >>Pathways in cancer, >>Primary immunodeficiency |
| Reactivity | Human,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-7R/CD127 around the phosphorylation site of Tyr449. AA range:410-459 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell,Epithelium,Spleen,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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