ABM98892
Septin 14 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Septin 14 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Septin 14 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Septin 14.
Background
SEPT14 is a member of the highly conserved septin family of GTP-binding cytoskeletal proteins implicated in membrane transport, apoptosis, cell polarity, cell cycle regulation, cytokinesis, and other cellular functions (Peterson et al., 2007 [PubMed 17922164]).[supplied by OMIM, Jul 2008],
Additional antibody information
| Specificity | Septin 14 Polyclonal Antibody detects endogenous levels of Septin 14 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Septin 14 Polyclonal Antibody | Western blot analysis of SEPT14 Antibody. The lane on the right is blocked with the SEPT14 peptide. | Western blot analysis of the lysates from Jurkat cells using SEPT14 antibody. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: Septin 14
| Target | Septin 14 |
| Also known as | SEPT14; Septin-14 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 50kD |
| Cellular localization | Cytoplasm . Cytoplasm, cytoskeleton . Cell projection, axon . Cell projection, dendrite . Perikaryon . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, acrosome . Colocalizes with actin stress fibers (PubMed:17922164). Expressed in the perinuclear rim and manchette structure in early elongating spermatids during spermiogenesis (By similarity). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SEPT14. AA range:341-390 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Septin 14
Also known as
SEPT14; Septin-14
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human SEPT14. AA range:341-390
Observed band
50kD
Cellular localization
Cytoplasm . Cytoplasm, cytoskeleton . Cell projection, axon . Cell projection, dendrite . Perikaryon . Cytoplasm, perinuclear region . Cytoplasmic vesicle, secretory vesicle, acrosome . Colocalizes with actin stress fibers (PubMed:17922164). Expressed in the perinuclear rim and manchette structure in early elongating spermatids during spermiogenesis (By similarity). .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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