ABM98882
Synaptotagmin X rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Synaptotagmin X rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Synaptotagmin X rabbit pAb is an ELK Biotechnology antibody for research workflows involving Synaptotagmin X.
Background
cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.,function:May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.,similarity:Belongs to the synaptotagmin family.,similarity:Contains 2 C2 domains.,subunit:Homodimer. Can also form heterodimers.,tissue specificity:Expressed only in pancreas, lung and kidney.,
Additional antibody information
| Specificity | Synaptotagmin X Polyclonal Antibody detects endogenous levels of Synaptotagmin X protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293 cells using Synaptotagmin X Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: Synaptotagmin X
| Target | Synaptotagmin X |
| Also known as | SYT10; Synaptotagmin-10; Synaptotagmin X; SytX |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 55kD |
| Cellular localization | Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Localizes to neuronal vesicles containing IGF1 that are not enriched at synapses. Does not colocalize with synaptic vesicles or with the Golgi apparatus. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | Synthesized peptide derived from Synaptotagmin X . at AA range: 380-460 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Synaptotagmin X
Also known as
SYT10; Synaptotagmin-10; Synaptotagmin X; SytX
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from Synaptotagmin X . at AA range: 380-460
Observed band
55kD
Cellular localization
Cytoplasmic vesicle, secretory vesicle membrane ; Single-pass membrane protein . Localizes to neuronal vesicles containing IGF1 that are not enriched at synapses. Does not colocalize with synaptic vesicles or with the Golgi apparatus. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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