Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98875

Anoctamin-9 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Anoctamin-9 rabbit pAb from ELK Biotechnology Read more ›

Anoctamin-9 rabbit pAb
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Anoctamin-9 rabbit pAb

Product Description

Anoctamin-9 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Anoctamin-9 in IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:May act as a calcium-activated chloride channel.,sequence caution:Intron retention.,similarity:Belongs to the anoctamin family.,

Additional antibody information

Specificity Anoctamin-9 Polyclonal Antibody detects endogenous levels of Anoctamin-9 protein.
Validation evidence captions Immunofluorescence analysis of HepG2 cells, using TM16J Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TM16J Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: Anoctamin-9
Collaborator ELK Biotechnology
Partner model ES5809
Target Anoctamin-9
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human TM16J. AA range:481-530
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Salivary gland,Spleen,Thymus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Anoctamin-9
Also known as
ANO9; PIG5; TMEM16J; TP53I5; Anoctamin-9; Transmembrane protein 16J; Tumor protein p53-inducible protein 5; p53-induced gene 5 protein
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human TM16J. AA range:481-530
Cellular localization
Cell membrane ; Multi-pass membrane protein . Shows predominantly an intracellular localization with a weak expression in the cell membrane.
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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