Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98872

SR-6 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SR-6 rabbit pAb from ELK Biotechnology Read more ›

SR-6 rabbit pAb
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SR-6 rabbit pAb

Product Description

SR-6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SR-6 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein that belongs to the seven-transmembrane G protein-coupled receptor family of proteins. The encoded protein couples with the Gs alpha subunit and stimulates adenylate cyclase to activate the cyclic AMP-dependent signaling pathway. This receptor is thought to regulate cholinergic neuronal transmission in the brain. Several antidepressants and antipsychotic drugs have a high affinity for this receptor. [provided by RefSeq, Aug 2013],

Additional antibody information

Specificity SR-6 Polyclonal Antibody detects endogenous levels of SR-6 protein.
Validation evidence captions Western Blot analysis of A549 cells using SR-6 Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: SR-6
Collaborator ELK Biotechnology
Partner model ES5806
Target SR-6
Research areas>>Calcium signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Serotonergic synapse
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human 5-HT-6. AA range:221-270
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in several human brain regions, most prominently in the caudate nucleus.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
SR-6
Also known as
HTR6; 5-hydroxytryptamine receptor 6; 5-HT-6; 5-HT6; Serotonin receptor 6
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human 5-HT-6. AA range:221-270
Observed band
47kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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