ABM98830
Fibulin-4 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Fibulin-4 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Fibulin-4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Fibulin-4.
Background
A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011],
Additional antibody information
| Specificity | Fibulin-4 Polyclonal Antibody detects endogenous levels of Fibulin-4 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using EFEMP2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using EFEMP2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 and NIH/3T3 cells, using EFEMP2 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: Fibulin-4
| Target | Fibulin-4 |
| Also known as | EFEMP2; FBLN4; EGF-containing fibulin-like extracellular matrix protein 2; Fibulin-4; FIBL-4; Protein UPH1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 50kD |
| Cellular localization | Secreted, extracellular space, extracellular matrix . Secreted, extracellular space, extracellular matrix, basement membrane . Localizes on the microfibrils surrounding ELN cores. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EFEMP2. AA range:91-140 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Fibulin-4
Also known as
EFEMP2; FBLN4; EGF-containing fibulin-like extracellular matrix protein 2; Fibulin-4; FIBL-4; Protein UPH1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human EFEMP2. AA range:91-140
Observed band
50kD
Cellular localization
Secreted, extracellular space, extracellular matrix . Secreted, extracellular space, extracellular matrix, basement membrane . Localizes on the microfibrils surrounding ELN cores. .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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