Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98815

NMDAε4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NMDAε4 rabbit pAb from ELK Biotechnology Read more ›

NMDAε4 rabbit pAb
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NMDAε4 rabbit pAb

Product Description

NMDAε4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GRIN2D in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). [provided by RefSeq, Mar 2010],

Additional antibody information

Specificity NMDAε4 Polyclonal Antibody detects endogenous levels of NMDAε4 protein.
Validation evidence captions Western Blot analysis of 293T cells using NMDAε4 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from COS7 cells, using GRIN2D Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: GRIN2D
Collaborator ELK Biotechnology
Partner model ES5662
Target GRIN2D
Research areas>>Calcium signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Circadian entrainment, >>Long-term potentiation, >>Glutamatergic synapse, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Spinocerebellar ataxia, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Cocaine addiction, >>Amphetamine addiction, >>Nicotine addiction, >>Alcoholism
Reactivity Human,Mouse,Rat,Monkey
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GRIN2D. AA range:671-720
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Fetal brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GRIN2D
Also known as
GRIN2D; GluN2D; NMDAR2D; Glutamate NMDA receptor subunit epsilon-4; EB11; N-methyl D-aspartate receptor subtype 2D; NMDAR2D; NR2D
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GRIN2D. AA range:671-720
Observed band
170kD
Cellular localization
Cell membrane ; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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