NMDAε2 (phospho Tyr1474) rabbit pAb from ELK Biotechnology Read more ›
NMDAε2 (phospho Tyr1474) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NMDAR2B in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-NMDAε2 (Y1474) Polyclonal Antibody detects endogenous levels of NMDAε2 protein only when phosphorylated at Y1474. |
|---|---|
| Validation evidence captions | Western blot analysis of MOUSE-BRAIN using p-NMDAε2 (Y1474) antibody. Antibody was diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NMDAR2B (Phospho-Tyr1474) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR2B (Phospho-Tyr1474) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from Jurkat cells treated with UV 15', using NMDAR2B (Phospho-Tyr1474) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5655 |
| Target | NMDAR2B |
| Research areas | >>Ras signaling pathway, >>Rap1 signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Circadian entrainment, >>Long-term potentiation, >>Glutamatergic synapse, >>Dopaminergic synapse, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Cocaine addiction, >>Amphetamine addiction, >>Nicotine addiction, >>Alcoholism, >>Systemic lupus erythematosus |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NMDAR2B around the phosphorylation site of Tyr1474. AA range:1435-1484 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
