NMDAε1/2 rabbit pAb from ELK Biotechnology Read more ›
NMDAε1/2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NMDAε1/2 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
| Specificity | NMDAε1/2 Polyclonal Antibody detects endogenous levels of NMDAε1/2 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HUVEC cells, using NMDAR2A/B Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NMDAR2A/B Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5653 |
| Target | NMDAε1/2 |
| Research areas | >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Circadian entrainment, >>Long-term potentiation, >>Glutamatergic synapse, >>Dopaminergic synapse, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Spinocerebellar ataxia, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Cocaine addiction, >>Amphetamine addiction, >>Nicotine addiction, >>Alcoholism, >>Systemic lupus erythematosus |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NMDAR2A/B. AA range:1216-1265 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Cerebellum,Epithelium,Hippocampus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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