ABM98811
NMDAε1/2 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
NMDAε1/2 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
NMDAε1/2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving NMDAε1/2.
Background
This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
Additional antibody information
| Specificity | NMDAε1/2 Polyclonal Antibody detects endogenous levels of NMDAε1/2 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HUVEC cells, using NMDAR2A/B Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using NMDAR2A/B Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: NMDAε1/2
| Target | NMDAε1/2 |
| Also known as | GRIN2A; NMDAR2A; Glutamate NMDA receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A; GRIN2B; NMDAR2B; Glutamate NMDA receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; N |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Cellular localization | Cell projection, dendritic spine . Cell membrane ; Multi-pass membrane protein . Cell junction, synapse . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane . Expression at the dendrite cell membrane and at synapses is regulated by SORCS2 and the retromer complex. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Circadian entrainment;>>Long-term potentiation;>>Glutamatergic synapse;>>Dopaminergic synapse;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Spinocerebellar ataxia;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Cocaine addiction;>>Amphetamine addiction;>>Nicotine addiction;>>Alcoholism;>>Systemic lupus erythematosus |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NMDAR2A/B. AA range:1216-1265 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
NMDAε1/2
Also known as
GRIN2A; NMDAR2A; Glutamate NMDA receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A; GRIN2B; NMDAR2B; Glutamate NMDA receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; N
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
IHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NMDAR2A/B. AA range:1216-1265
Cellular localization
Cell projection, dendritic spine . Cell membrane ; Multi-pass membrane protein . Cell junction, synapse . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane . Expression at the dendrite cell membrane and at synapses is regulated by SORCS2 and the retromer complex. .
Dilution range
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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