ABM98808
NMDAζ1 (phospho Ser896) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
NMDAζ1 (phospho Ser896) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
NMDAζ1 (phospho Ser896) rabbit pAb is an ELK Biotechnology antibody for research workflows involving NMDAR1.
Background
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-NMDAζ1 (S896) Polyclonal Antibody detects endogenous levels of NMDAζ1 protein only when phosphorylated at S896. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T using Phospho-NMDAζ1 (S896) Polyclonal Antibody. Antibody was diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NMDAR1 (Phospho-Ser896) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR1 (Phospho-Ser896) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from K562 cells treated with PMA 125ng/ml 30', using NMDAR1 (Phospho-Ser896) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
Product type
Primary Antibody›
Target: NMDAR1
| Target | NMDAR1 |
| Also known as | GRIN1; NMDAR1; Glutamate NMDA receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 105kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane . Cell junction, synapse, postsynaptic density . Enriched in postsynaptic plasma membrane and postsynaptic densities. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Circadian entrainment;>>Long-term potentiation;>>Glutamatergic synapse;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Cocaine addiction;>>Amphetamine addiction;>>Nicotine addiction;>>Alcoholism |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NMDAR1 around the phosphorylation site of Ser896. AA range:862-911 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
NMDAR1
Also known as
GRIN1; NMDAR1; Glutamate NMDA receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NMDAR1 around the phosphorylation site of Ser896. AA range:862-911
Observed band
105kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane . Cell junction, synapse, postsynaptic density . Enriched in postsynaptic plasma membrane and postsynaptic densities. .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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