NMDAζ1 (phospho Ser896) rabbit pAb from ELK Biotechnology Read more ›
NMDAζ1 (phospho Ser896) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NMDAR1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-NMDAζ1 (S896) Polyclonal Antibody detects endogenous levels of NMDAζ1 protein only when phosphorylated at S896. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T using Phospho-NMDAζ1 (S896) Polyclonal Antibody. Antibody was diluted at 1:500 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NMDAR1 (Phospho-Ser896) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR1 (Phospho-Ser896) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from K562 cells treated with PMA 125ng/ml 30', using NMDAR1 (Phospho-Ser896) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5646 |
| Target | NMDAR1 |
| Research areas | >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Circadian entrainment, >>Long-term potentiation, >>Glutamatergic synapse, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Cocaine addiction, >>Amphetamine addiction, >>Nicotine addiction, >>Alcoholism |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NMDAR1 around the phosphorylation site of Ser896. AA range:862-911 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Cerebellum,Hippocampus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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