Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98797

GPR42 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GPR42 rabbit pAb from ELK Biotechnology Read more ›

GPR42 rabbit pAb
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GPR42 rabbit pAb

Product Description

GPR42 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GPR42 in IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

GPR42, also named as GPR42P, FFAR1L and GPR41L, belongs to the G-protein coupled receptor 1 family. GPR42 appears to have arisen by tandem duplication of FFAR3 and have lost the ability to activate G-protein family in respose to carboxylate ligands primarily due to an amino acid change at position 174. It is an orphan receptor.

Additional antibody information

Specificity GPR42 Polyclonal Antibody detects endogenous levels of GPR42 protein.
Validation evidence captions Immunofluorescence analysis of MCF-7 cells, using GPR42 Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: GPR42
Collaborator ELK Biotechnology
Partner model ES5619
Target GPR42
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GPR42. AA range:12-61
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
Human Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GPR42
Also known as
GPR42; GPR42P; G-protein coupled receptor 42
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR42. AA range:12-61
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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