GPR40 rabbit pAb from ELK Biotechnology Read more ›
GPR40 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GPR40 in WB, IF, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009],
| Specificity | GPR40 Polyclonal Antibody detects endogenous levels of GPR40 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of COS7 cells using GPR40 Polyclonal Antibody diluted at 1:500 | Western Blot analysis of COS-7 cells using GPR40 Polyclonal Antibody diluted at 1:500 | Western blot analysis of Mouse-kidney lysis using GPR40 antibody. Antibody was diluted at 1:500 | Immunofluorescence analysis of LOVO cells, using FFAR1 Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5617 |
| Target | GPR40 |
| Research areas | >>Insulin secretion |
| Reactivity | Human,Monkey |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FFAR1. AA range:185-234 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Detected in brain and pancreas. Detected in pancreatic beta cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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