Cadherin-20 rabbit pAb
Cadherin-20 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cadherin-20 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cadherin-20.
Background
This gene is a type II classical cadherin from the cadherin superfamily and one of three cadherin 7-like genes located in a cluster on chromosome 18. The encoded membrane protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Since disturbance of intracellular adhesion is a prerequisite for invasion and metastasis of tumor cells, cadherins are considered prime candidates for tumor suppressor genes. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Cadherin-20 Polyclonal Antibody detects endogenous levels of Cadherin-20 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of CDH20 Antibody. The lane on the right is blocked with the CDH20 peptide. | Immunohistochemistryt analysis of paraffin-embedded human brain, using CDH20 Antibody. The lane on the right is blocked with the CDH20 peptide. | Immunofluorescence analysis of NIH/3T3 cells, using CDH20 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDH20 Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Cadherin-20 |
| Also known as | CDH20; CDH7L3; Cadherin-20 |
| Concentration | 1 mg/ml |
| Working concentration | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Cellular localization | Cell membrane; Single-pass type I membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIFELISA |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDH20. AA range:111-160 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.