Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98769

AQP11 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

AQP11 rabbit pAb from ELK Biotechnology Read more ›

AQP11 rabbit pAb
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AQP11 rabbit pAb

Product Description

AQP11 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AQP11 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

domain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,function:Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family. AQP11/AQP12 subfamily.,tissue specificity:Expressed at low levels in roots and leaves.,

Additional antibody information

Specificity AQP11 Polyclonal Antibody detects endogenous levels of AQP11 protein.
Validation evidence captions Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: AQP11
Collaborator ELK Biotechnology
Partner model ES5575
Target AQP11
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from AQP11 . at AA range: 130-210
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Detected in the sperm head and tail (at protein level) (PubMed:28042826). Expressed in subcutaneous adipocytes (PubMed:24845055). Expressed in testis, kidney and ejaculated spermatozoa (PubMed:19812234).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
AQP11
Also known as
AQP11; AQPX1; PSEC0027; Aquaporin-11; AQP-11
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from AQP11 . at AA range: 130-210
Observed band
26kD
Cellular localization
Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein . Cytoplasm . Cytoplasm, perinuclear region . Localizes mainly to the periphery of lipid droplets (PubMed:24845055). it accumulates partly in mitochondrial-associated endoplasmic reticulum membranes (PubMed:31546170). .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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