GnRH-R rabbit pAb
GnRH-R rabbit pAb from ELK Biotechnology Read more ›
Product Description
GnRH-R rabbit pAb is an ELK Biotechnology antibody for research workflows involving GnRH-R.
Background
This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gen
Additional antibody information
| Specificity | GnRH-R Polyclonal Antibody detects endogenous levels of GnRH-R protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using GNRHR Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | GnRH-R |
| Also known as | GNRHR; GRHR; Gonadotropin-releasing hormone receptor; GnRH receptor; GnRH-R |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 37kD |
| Cellular localization | Cell membrane; Multi-pass membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Neuroactive ligand-receptor interaction;>>GnRH signaling pathway |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GNRHR. AA range:41-90 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.