Gα i-3 rabbit pAb from ELK Biotechnology Read more ›
Gα i-3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GNAI3 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012],
| Specificity | Gα i-3 Polyclonal Antibody detects endogenous levels of Gα i-3 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night |
| Collaborator | ELK Biotechnology |
| Partner model | ES5567 |
| Target | GNAI3 |
| Research areas | >>Rap1 signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Axon guidance, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>Leukocyte transendothelial migration, >>Circadian entrainment, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>Serotonergic synapse, >>GABAergic synapse, >>Dopaminergic synapse, >>Long-term depression, >>Progesterone-mediated oocyte maturation, >>Estrogen signaling pathway, >>Melanogenesis, >>Oxytocin signaling pathway, >>Regulation of lipolysis in adipocytes, >>Renin secretion, >>Relaxin signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Cushing syndrome, >>Growth hormone synthesis, >>Gastric acid secretion, >>Parkinson disease, >>Cocaine addiction, >>Morphine addiction, >>Alcoholism, >>Pertussis, >>Chagas disease, >>Toxoplasmosis, >>Human cytomegalovirus infectio |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GNAI3. AA range:31-80 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Liver, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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