Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98763

GlyRβ rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GlyRβ rabbit pAb from ELK Biotechnology Read more ›

GlyRβ rabbit pAb
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GlyRβ rabbit pAb

Product Description

GlyRβ rabbit pAb is a research-use antibody from ELK Biotechnology for studying GlyRβ in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

Additional antibody information

Specificity GlyRβ Polyclonal Antibody detects endogenous levels of GlyRβ protein.
Validation evidence captions Western blot analysis of GLRB Antibody. The lane on the right is blocked with the GLRB peptide. | Western blot analysis of the lysates from HUVECcells using GLRB antibody.

Key Facts

Product typePrimary Antibody Target: GlyRβ
Collaborator ELK Biotechnology
Partner model ES5566
Target GlyRβ
Research areas>>Neuroactive ligand-receptor interaction
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GLRB. AA range:211-260
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Hippocampus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GlyRβ
Also known as
GLRB; Glycine receptor subunit beta; Glycine receptor 58 kDa subunit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GLRB. AA range:211-260
Observed band
56kD
Cellular localization
Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell junction, synapse . Cell projection, dendrite . Cell membrane ; Multi-pass membrane protein . Cytoplasm . Retained in the cytoplasm upon heterologous expression by itself. Coexpression with GPHN promotes expression at the cell membrane (PubMed:12684523). Coexpression with GLRA1, GLRA2 or GLRA3 promotes expression at the cell membrane. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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