Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98751

Connexin 37 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Connexin 37 rabbit pAb from ELK Biotechnology Read more ›

Connexin 37 rabbit pAb
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Connexin 37 rabbit pAb

Product Description

Connexin 37 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Connexin 37 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Connexin 37 Polyclonal Antibody detects endogenous levels of Connexin 37 protein.
Validation evidence captions Western blot analysis of lysates from Jurkat cells, using GJA4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from 293 cells using GJA4 antibody.

Key Facts

Product typePrimary Antibody Target: Connexin 37
Collaborator ELK Biotechnology
Partner model ES5528
Target Connexin 37
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GJA4. AA range:81-130
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Connexin 37
Also known as
GJA4; Gap junction alpha-4 protein; Connexin-37; Cx37
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GJA4. AA range:81-130
Observed band
37kD
Cellular localization
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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