p52 S6 kinase rabbit pAb from ELK Biotechnology Read more ›
p52 S6 kinase rabbit pAb is a research-use antibody from ELK Biotechnology for studying p52 S6 kinase in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:Instead of Lys-820, Arg-820 is found at the binding site.,domain:The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.,function:May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 MIT domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Also found in some small dot-like or ring-shaped early endosome structures.,subunit:Interacts with SPHK1 and phosphatidylinositol 3-phosphate.,tissue specificity:Highly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon.,
| Specificity | p52 S6 kinase Polyclonal Antibody detects endogenous levels of p52 S6 kinase protein. |
|---|---|
| Validation evidence captions | Western blot analysis of HELA 293T lysis using p52 S6 kinase antibody. Antibody was diluted at 1:500 | Immunofluorescence analysis of LOVO cells, using RPS6KC1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using RPS6KC1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using RPS6KC1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5514 |
| Target | p52 S6 kinase |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPS6KC1. AA range:231-280 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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