Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98733

GBP1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GBP1 rabbit pAb from ELK Biotechnology Read more ›

GBP1 rabbit pAb
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GBP1 rabbit pAb

Product Description

GBP1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GBP1 in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Guanylate binding protein expression is induced by interferon. Guanylate binding proteins are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity GBP1 Polyclonal Antibody detects endogenous levels of GBP1 protein.
Validation evidence captions Immunofluorescence analysis of MCF7 cells, using GBP1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HUVEC cells, using GBP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: GBP1
Collaborator ELK Biotechnology
Partner model ES5491
Target GBP1
Research areas>>NOD-like receptor signaling pathway
Reactivity Human,Rat,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GBP1. AA range:71-120
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Placenta,Spleen,Uterus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GBP1
Also known as
GBP1; Interferon-induced guanylate-binding protein 1; GTP-binding protein 1; GBP-1; HuGBP-1; Guanine nucleotide-binding protein 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GBP1. AA range:71-120
Observed band
68kD
Cellular localization
Cytoplasm . Golgi apparatus membrane; Lipid-anchor ; Cytoplasmic side. Cell membrane . Secreted . Cytoplasmic vesicle . Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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